For DNA sequencing high GC regions and rapid sequencing from nanogram amounts of DNA templates.

Description:
A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment).
 

Bst.jpg"
Figure: MCLAB Bst DNA Polymerase (large fragment) shows higher strand -displacing polymerase activity than two other vendors' products.

Application:
- Sequencing through problematic secondary structures

Source:
Bst DNA Polymerase (large fragment) is a portion of Bacillus stearothermophilus DNA Polymerase protein that contains 5´-> 3´ polymerase activity, but lacks 5'->3' and 3'->5' exonuclease activities.

Specific Activity: 120,000 U/mg

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Reference:
Kiefer, et al. Structure 15 January 1997. 5, 95-108.